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KMID : 0364320180430020019
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Journal of East-West Medicine
2018 Volume.43 No. 2 p.19 ~ p.28
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Protective effect of licorice on the cantharides-treated hepatic cell toxicity
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Ha Seong-Ho
Kim Sang-Chan
Park Sook-Jahr
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Abstract
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Objective : Cantharides, the dried medicinal insect, has been used to treat seminal weakness and chronic inflammation of the bladder. However, cantharides is irritating and toxic when used in large amounts. This study investigated the effects of licorice on hepatic cell toxicity induced by high dose of cantharides.
Methods : Cantharides and licorice were prepared by extracting 200 g of dry samples in 1.5ℓ of water for 3 h. MTT assay was used to determine cell viability. After culture, the cells were stained with MTT solution for 4 h. The dark blue crystals of formazan were dissolved with dimethylsulfoxide, and the absorbance of the formazan solution was measured at 570 nm.
Results : Cantharides showed strong cytotoxic activity in hepatic cells. However, cotreatment of licorice markedly recovered the number of viable cells compared to those samples without treatment. Furthermore, we examined the hepatoprotective effect of the major components of licorice, glycyrrhizind (Gly), isoliquiritigenin (ILQ) and liquiritigenin (LQ). Of the three major compounds, ILQ and LQ showed significant cytoprotective effects.
Conclusion : These results provide evidence that objectively explains the role of licorice, which has long been used for detoxification purposes in Korean medicine.
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KEYWORD
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cantharides, licorice, isoliquiritigenin, hepatoprotective effect
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